Our Research Topics
Since 2013, our research has focused on the CRISPR bacterial "immune system" nucleases. Our research priorities are to develop gene editing systems based on these nucleases, to increase their efficiency, to optimize them and to understand their function. Specifically:
⁃ We are investigating the off-target activity of the SpCas9 nuclease (Nat Commun, 2023), and creating a general off-target-free editing procedure.
⁃ We are developing SpCas9 nuclease variants with increased precision (Genome Biol, 2017; Nat Commun, 2020; Nat Commun, 2021; Nat Commun, 2022).
⁃ We are exploring the motif and sequence preferences of SpCas9 nuclease and our variants (Nucleic Acids Res, 2021; Nucleic Acids Res, 2023).
⁃ We are continuously developing Cas12a variants that are more efficient and have different PAM binding specificities compared to the wild-type protein (Nucleic Acids Res, 2018; Nucleic Acids Res, 2020).
⁃ For all of this, we have developed several different, widely applicable reporter systems. Such is the BEAR and PEAR system suitable for testing the effectiveness of base and primary editors (Nat Commun, 2021; eLife, 2022), as well as the GFxFP reporter system for testing nuclease activity independent of genomic context (Biol Direct, 2016).
News
ProPE ON TELEX
The results of our recently published study were also highlighted in the Telex magazine.
ProPE PLANNER NOW AVAILABLE
The ProPE Planner tool, which enables the design of eng- and tpg-RNA pairs, is now available and ready to use.
Our latest publication is out now
We won the NKKP Excellence grant
Our project entitled "Development of new gene editing tools" received HUF 200 million in funding for two years from the National Research Excellence Program (NKKP).
