Éva Varga
Predoc
The focus of my research topic is the Cas12a (formerly known as Cpf1) protein belonging to the CRISPR-Cas system. It is an excellent alternative to the widely recognized Cas9 protein, offering enhanced effectiveness for specific applications due to its unique properties. However, like all systems, it does have its limitations. I am actively working to minimize these limitations to expand its potential for broader and more effective use.
Education
2018 - Present
Doctoral school
Multidisciplinary Medical Sciences
University of Szeged (SZTE)
2018
Biology MSc
Molecular, Immune and Microbiology specialization
Eötvös Loránd University (ELTE)
Research Interest
My primary interest lies in the treatment of genetic diseases, which inspired me to focus on the development of the CRISPR system. I aspire to contribute to something meaningful, enhancing both the field and my own utility in it. The CRISPR system has proven, especially in recent years, to offer solutions for many previously untreatable diseases and to improve patients' quality of life. Therefore, advancing this technology is crucial for its widespread application. Alongside my colleagues, I have participated in numerous related projects, with my work on Cas12a proteins being particularly significant to me. I thoroughly enjoy laboratory work, especially cell manipulation, but I also find the analysis of next-generation sequencing (NGS) data to be both fascinating and enjoyable.
Publications
Huszár, Krisztina ; Welker, Zsombor ; Györgypál, Zoltán ; Tóth, Eszter ; Ligeti, Zoltán ; Kulcsár, Péter István ; Dancsó, János ; Tálas, András ; Krausz, Sarah Laura ; Varga, Éva ; Ervin Welker
Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
NUCLEIC ACIDS RESEARCH 51 : 11 pp. 5847-5863. , 17 p. (2023)
DOI WoS REAL Scopus PubMed
Article (Journal Article)
Tálas, András ; Simon, Dorottya Anna ; Kulcsár, Péter István ; Varga, Éva ; Krausz, Sarah Laura ; Welker, Ervin
BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
NATURE COMMUNICATIONS 12 : 1 Paper: 6353 , 14 p. (2021)
DOI WoS REAL Scopus PubMed
Article (Journal Article)
Tálas, András ; Huszár, Krisztina ; Kulcsár, Péter István ; Varga, Julia K ; Varga, Éva ; Tóth, Eszter ; Welker, Zsombor ; Erdős, Gergely ; Pach, Péter Ferenc ; Welker, Agnes ; Györgypál, Zoltán ; Tusnády, Gábor ; Welker, Ervin
A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs
NUCLEIC ACIDS RESEARCH 49 : 6 Paper: e31 , 12 p. (2021)
DOI WoS REAL Scopus PubMed
Article (Journal Article)
Tóth, Eszter ; Varga, Éva ; Kulcsár, Péter István ; Kocsis-Jutka, Virág ; Krausz, Sarah Laura ; Nyeste, Antal ; Welker, Zsombor ; Huszár, Krisztina ; Ligeti, Zoltán ; Tálas, András ; Welker, Ervin
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases
NUCLEIC ACIDS RESEARCH 48 : 7 pp. 3722-3733. , 12 p. (2020)
DOI REAL WoS Scopus PubMed Other URLs
Article (Journal Article)
Tóth, Eszter ; Czene, Bernadette C ; Kulcsár, Péter István ; Krausz, Sarah Laura ; Tálas, András ; Nyeste, Antal ; Varga, Éva ; Huszár, Krisztina ; Weinhardt, Nóra ; Ligeti, Zoltán ; Borsy, Adrienn Éva ; Fodor, Elfrieda ; Welker, Ervin
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
NUCLEIC ACIDS RESEARCH 46 : 19 pp. 10272-10285. , 14 p. (2018)
DOI REAL WoS Scopus PubMed
Article (Journal Article)