Krisztina Huszár
Predoc
My research interests are focused on the molecular mechanisms of CRISPR-Cas9 systems, with a particular emphasis on the Streptococcus pyogenes Cas9 (SpCas9) nuclease. My research aims to gain a deeper understanding of the sequence-dependent preferences and intrinsic interactions of sgRNA that influence Cas9 function. In particular, I am interested in the role of intramolecular interactions between the spacer and scaffold regions that inhibit sgRNA loading and target DNA cleavage. By gaining a better understanding of these interactions, we can optimize CRISPR-based gene editing tools.
Education
Present
Doctoral School of Biology
Classical and molecular genetics
Eötvös Loránd University (ELTE)
2007 – 2009
Biologist MSc
Molecular Biology - Genetics specialization
Eötvös Loránd University (ELTE)
Publications
Huszár, Krisztina ; Welker, Zsombor ; Györgypál, Zoltán ; Tóth, Eszter ; Ligeti, Zoltán ; Kulcsár, Péter István ; Dancsó, János ; Tálas, András ; Krausz, Sarah Laura ; Varga, Éva ; Ervin Welker
Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
NUCLEIC ACIDS RESEARCH 51 : 11 pp. 5847-5863. , 17 p. (2023)
DOI WoS REAL Scopus PubMed
Article (Journal Article)
Kulcsár, Péter István ; Tálas, András ; Ligeti, Zoltán ; Tóth, Eszter ; Rakvács, Zsófia ; Bartos, Zsuzsanna ; Krausz, Sarah Laura ; Welker, Agnes ; Végi, Vanessa Laura ; Huszár, Krisztina ; Ervin Welker
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
NATURE COMMUNICATIONS 14 : 1 Paper: 5746 , 20 p. (2023)
DOI WoS Scopus PubMed
Article (Journal Article)
Wachtl, Gerda ; Schád, Éva ; Huszár, Krisztina ; Palazzo, Antonio ; Ivics, Zoltán ; Tantos, Ágnes ; Orbán, Tamás I.
Functional Characterization of the N-Terminal Disordered Region of the piggyBac Transpose
International Journal of Molecular Sciences 23, no. 18: 10317. (2022)
DOI PubMed
Tálas, András ; Huszár, Krisztina ; Kulcsár, Péter István ; Varga, Julia K ; Varga, Éva ; Tóth, Eszter ; Welker, Zsombor ; Erdős, Gergely ; Pach, Péter Ferenc ; Welker, Agnes ; Györgypál, Zoltán ; Tusnády, Gábor ; Welker, Ervin
A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs
NUCLEIC ACIDS RESEARCH 49 : 6 Paper: e31 , 12 p. (2021)
DOI WoS REAL Scopus PubMed
Article (Journal Article)
Tóth, Eszter ; Varga, Éva ; Kulcsár, Péter István; Kocsis-Jutka, Virág ; Krausz, Sarah Laura ; Nyeste, Antal ; Welker, Zsombor ; Huszár, Krisztina ; Ligeti, Zoltán ; Tálas, András ; Welker, Ervin
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases
NUCLEIC ACIDS RESEARCH 48 : 7 pp. 3722-3733. , 12 p. (2020)
DOI REAL WoS Scopus PubMed Other URLs
Article (Journal Article)
Sangeetham, Sudheer Babu ; Huszár, Krisztina ; Bencsura, Petra ; Nyeste, Antal ; Hunyadi-Gulyas, Éva ; Fodor, Elfrieda ; Welker, Ervin
Interrogating the Dimerization Interface of the Prion Protein Via Site-Specific Mutations to p-Benzoyl-L-Phenylalanine
JOURNAL OF MOLECULAR BIOLOGY 430 : 17 pp. 2784-2801. , 18 p. (2018)
DOI WoS REAL Scopus PubMed Other URLs
Article (Journal Article)
Tóth, Eszter ; Czene, Bernadette C ; Kulcsár, Péter István ; Krausz, Sarah Laura ; Tálas, András ; Nyeste, Antal ; Varga, Éva ; Huszár, Krisztina ; Weinhardt, Nóra ; Ligeti, Zoltán ; Borsy, Adrienn Éva ; Fodor, Elfrieda ; Welker, Ervin
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
NUCLEIC ACIDS RESEARCH 46 : 19 pp. 10272-10285. , 14 p. (2018)
DOI REAL WoS Scopus PubMed
Article (Journal Article)
Kulcsár, Péter István ; Tálas, András ; Huszár, Krisztina ; Ligeti, Zoltán ; Toth, Eszter ; Weinhardt, Nóra ; Fodor, Elfrieda ; Welker, Ervin
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
GENOME BIOLOGY 18 Paper: 190 , 17 p. (2017)
DOI REAL WoS Scopus PubMed SZTE Publicatio
Article (Journal Article)
Tálas, András ; Kulcsár, Péter István ; Weinhardt, Nóra ; Borsy, Adrienn ; Tóth, Eszter ; Szebényi, Kornélia ; Krausz, Sarah Laura ; Huszár, Krisztina ; Vida, István ; Sturm, Ádám ; Gordos, Bianka ; Hoffmann, Orsolya Ivett ; Bencsura, Petra ; Nyeste, Antal ; Ligeti, Zoltán ; Fodor, Elfrieda ; Welker, Ervin
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid
DNA RESEARCH 24 : 6 pp. 609-621. , 13 p. (2017)
DOI REAL WoS EDIT Scopus PubMed SZTE Publicatio
Article (Journal Article)
Tóth, Eszter ; Weinhardt, Nóra ; Bencsura, Petra ; Huszár, Krisztina ; Kulcsár, Péter István ; Tálas, András ; Fodor, Elfrieda ; Welker, Ervin
Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells
BIOLOGY DIRECT 11 Paper: 46 , 14 p. (2016)
DOI REAL WoS Scopus PubMed SZTE Publicatio
Article (Journal Article)
Tóth, Eszter ; Huszár, Krisztina ; Bencsura, Petra ; Kulcsár, Péter István ; Vodicska, Barbara ; Nyeste, Antal ; Welker, Zsombor ; Tóth, Szilvia ; Welker, Ervin
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning.
PLOS ONE 9 : 3 Paper: e90896 , 12 p. (2014)
DOI WoS Scopus PubMed
Article (Journal Article)
Schäfer, Balázs ; Orbán, Erika ; Borics, Attila ; Huszár, Krisztina ; Nyeste, Antal ; Welker, Ervin; Tömböly, Csaba
Preparation of semisynthetic lipoproteins with fluorescent cholesterol anchor and their introduction to the cell membrane with minimal disruption of the membrane.
BIOCONJUGATE CHEMISTRY 24 : 10 pp. 1684-1697. , 14 p. (2013)
DOI REAL WoS Scopus PubMed
Article (Journal Article)