I am working to understand how a CRISPR-Cas9-based gene editing technique, prime editing, works in human cells. I am investigating the effects of the complementarities in the extended RNA used in this method, which could potentially reduce the efficiency of editing. I am also involved in the development of a prime editing-based method that allows efficient and accurate insertion of longer DNA sequences into the genome. This will allow the tagging of proteins without creating unwanted modifications in the coding gene and affecting the function of the gene.