{"id":30,"date":"2020-04-13T11:18:54","date_gmt":"2020-04-13T11:18:54","guid":{"rendered":"https:\/\/welkerlab.hu\/home\/"},"modified":"2025-11-05T17:31:38","modified_gmt":"2025-11-05T16:31:38","slug":"home","status":"publish","type":"page","link":"https:\/\/welkerlab.hu\/en\/","title":{"rendered":"Home"},"content":{"rendered":"
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"welker\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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ProPE PLANNER<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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A tool for designing eng- and tpgRNA pairs<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Our Team<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tMissing: Margit Dobr\u00e1di, Krisztina Jakab, Eszter T\u00f3th, P\u00e9ter T\u00f3th\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Our Research Topics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Since 2013, our research has focused on the CRISPR bacterial \"immune system\" nucleases. Our research priorities are to develop gene editing systems based on these nucleases, to increase their efficiency, to optimize them and to understand their function. Specifically:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u2043 We are investigating the off-target activity of the SpCas9 nuclease (Nat Commun, 2023<\/a>), and creating a general off-target-free editing procedure.
\u2043 We are developing SpCas9 nuclease variants with increased precision (Genome Biol, 2017<\/a>; Nat Commun, 2020<\/a>; Nat Commun, 2021<\/a>; Nat Commun, 2022<\/a>).
\u2043 We are exploring the motif and sequence preferences of SpCas9 nuclease and our variants (Nucleic Acids Res, 2021<\/a>;\u00a0 Nucleic Acids Res, 2023<\/a>).
\u2043 We are continuously developing Cas12a variants that are more efficient and have different PAM binding specificities compared to the wild-type protein (Nucleic Acids Res, 2018<\/a>; Nucleic Acids Res, 2020<\/a>).
\u2043 For all of this, we have developed several different, widely applicable reporter systems. Such is the BEAR and PEAR system suitable for testing the effectiveness of base and primary editors (Nat Commun, 2021<\/a>; eLife, 2022<\/a>), as well as the GFxFP reporter system for testing nuclease activity independent of genomic context (Biol Direct, 2016<\/a>).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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News<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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ProPE ON TELEX<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The results of our recently published study were also highlighted in the Telex magazine.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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ProPE PLANNER NOW AVAILABLE<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The ProPE Planner tool, which enables the design of eng- and tpg-RNA pairs, is now available and ready to use.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Our latest publication is out now <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tSarah Laura Krausz's work aims to increase the efficiency of prime editing. The publication was accepted by the Nature Catalysis.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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We won the NKKP Excellence grant<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Our project entitled \"Development of new gene editing tools\" received HUF 200 million in funding for two years from the National Research Excellence Program (NKKP).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t2025. 09. 20.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/a>\n\t\t
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Our undergraduate students have passed their final exams<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tBal\u00e1zs Csoma and P\u00e9ter T\u00f3th have successfully completed their BSc studies! We will continue our work together from September. Congratulations to them!\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t2024. 07. 30.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

ProPE PLANNER Eszk\u00f6z eng- \u00e9s tpg-RNS p\u00e1rok tervez\u00e9s\u00e9hez publik\u00e1ci\u00f3 megtekint\u00e9se CSapatunk A k\u00e9pr\u0151l hi\u00e1nyzik: Dobr\u00e1di Margit, Jakab Krisztina, T\u00f3th Eszter, T\u00f3th P\u00e9ter R\u00f3lunk Kutat\u00e1si T\u00e9m\u00e1ink 2013 \u00f3ta kutat\u00e1sunk k\u00f6z\u00e9ppontj\u00e1ban a CRISPR bakteri\u00e1lis \u201eimmunrendszer\u201d nukle\u00e1zai\u00e1llnak. Kiemelten foglalkozunk ezen nukle\u00e1zokon alapul\u00f3 g\u00e9nedit\u00e1l\u00f3 rendszerekfejleszt\u00e9s\u00e9vel, hat\u00e9konys\u00e1guk n\u00f6vel\u00e9s\u00e9vel, optimaliz\u00e1l\u00e1s\u00e1val \u00e9s m\u0171k\u00f6d\u00e9s\u00fck meg\u00e9rt\u00e9s\u00e9vel. Pontosabban: \u2043 Dolgozunk az SpCas9 nukle\u00e1z off-target aktivit\u00e1s\u00e1nak […]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"enabled","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-30","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/30","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/comments?post=30"}],"version-history":[{"count":481,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/30\/revisions"}],"predecessor-version":[{"id":3055,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/30\/revisions\/3055"}],"wp:attachment":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/media?parent=30"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}