{"id":2866,"date":"2025-03-07T10:42:51","date_gmt":"2025-03-07T09:42:51","guid":{"rendered":"https:\/\/welkerlab.hu\/?page_id=2866"},"modified":"2026-04-16T12:26:46","modified_gmt":"2026-04-16T10:26:46","slug":"pear","status":"publish","type":"page","link":"https:\/\/welkerlab.hu\/en\/pear\/","title":{"rendered":"PEAR"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2866\" class=\"elementor elementor-2866\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3feac28 e-flex e-con-boxed e-con e-parent\" data-id=\"3feac28\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-3c9ee12 e-con-full e-flex e-con e-child\" data-id=\"3c9ee12\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ead453d elementor-widget elementor-widget-heading\" data-id=\"ead453d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">prime editor activity reporter (PEAR)<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-94ae5c8 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"94ae5c8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-8ccb380 e-flex e-con-boxed e-con e-parent\" data-id=\"8ccb380\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-d19204f e-con-full e-flex e-con e-child\" data-id=\"d19204f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-594473d elementor-widget elementor-widget-text-editor\" data-id=\"594473d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tPrime editing is a CRISPR\/Cas9-based gene editing method that allows the integration of short insertions, deletions and substitutions into the genome. The prime editor activity reporter (PEAR) is a plasmid-based, sensitive, fluorescent reporter system for testing and comparing the activity of prime editors. The advantage of the PEAR reporter system is that it can be used to test editing efficiency independently of the genomic context.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-410408f elementor-widget elementor-widget-image\" data-id=\"410408f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"768\" height=\"630\" src=\"https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear-768x630.jpg\" class=\"attachment-medium_large size-medium_large wp-image-2757\" alt=\"\" srcset=\"https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear-768x630.jpg 768w, https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear-300x246.jpg 300w, https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear-1024x840.jpg 1024w, https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear-15x12.jpg 15w, https:\/\/welkerlab.hu\/wp-content\/uploads\/2025\/01\/pear.jpg 1500w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-05643a3 elementor-widget elementor-widget-text-editor\" data-id=\"05643a3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Principle of the prime editor activity reporter (PEAR) assay.<\/strong> (A) Schematic of the PEAR. The mechanism of PEAR is based on the same concept as BEAR, and it contains the same inactive splice site. (B) Optimization of PBS, RT, and complementary DNA strand nicking on the PEAR-GFP plasmid. C, D) Flow cytometry measurements of HEK293T cells transfected with active (positive control) or with inactive PEAR plasmids either along with nCas9 for negative controls or with PE2 vectors (black, gray, or green columns, respectively)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aedf1ac elementor-widget elementor-widget-text-editor\" data-id=\"aedf1ac\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Reference<\/strong><br \/>Simon, Dorottya Anna ; T\u00e1las, Andr\u00e1s ; Kulcs\u00e1r, P\u00e9ter Istv\u00e1n ; Bicz\u00f3k, Zsuzsanna ; Krausz, Sarah Laura ; V\u00e1rady, Gy\u00f6rgy ; Welker, Ervin<br \/><em>PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully primed edited cells<\/em><br \/>ELIFE 11 Paper: e69504 , 19 p. (2022)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dbca793 elementor-align-left elementor-widget elementor-widget-button\" data-id=\"dbca793\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm elementor-animation-grow\" href=\"https:\/\/elifesciences.org\/articles\/69504\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-angle-double-right\" viewbox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M224.3 273l-136 136c-9.4 9.4-24.6 9.4-33.9 0l-22.6-22.6c-9.4-9.4-9.4-24.6 0-33.9l96.4-96.4-96.4-96.4c-9.4-9.4-9.4-24.6 0-33.9L54.3 103c9.4-9.4 24.6-9.4 33.9 0l136 136c9.5 9.4 9.5 24.6.1 34zm192-34l-136-136c-9.4-9.4-24.6-9.4-33.9 0l-22.6 22.6c-9.4 9.4-9.4 24.6 0 33.9l96.4 96.4-96.4 96.4c-9.4 9.4-9.4 24.6 0 33.9l22.6 22.6c9.4 9.4 24.6 9.4 33.9 0l136-136c9.4-9.2 9.4-24.4 0-33.8z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">View publication<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>prime editor activity reporter (PEAR) A prime editing egy CRISPR\/Cas9 alap\u00fa g\u00e9nszerkeszt\u00e9si elj\u00e1r\u00e1s, amely lehet\u0151v\u00e9 teszi r\u00f6vid inszerci\u00f3k, del\u00e9ci\u00f3k \u00e9s szubsztit\u00faci\u00f3k bevitel\u00e9t a genomba. A prime editor activity reportert (PEAR) egy plazmid-alap\u00fa, \u00e9rz\u00e9keny, fluoreszcens riporter rendszer, amely a prime editorok akitivit\u00e1s\u00e1nak tesztel\u00e9s\u00e9re \u00e9s \u00f6sszehasonl\u00edt\u00e1s\u00e1ra szolg\u00e1l. A PEAR riporter rendszer el\u0151nye, hogy a genomi kontextust\u00f3l f\u00fcggetlen\u00fcl [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2866","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/comments?post=2866"}],"version-history":[{"count":13,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2866\/revisions"}],"predecessor-version":[{"id":3182,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2866\/revisions\/3182"}],"wp:attachment":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/media?parent=2866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}