{"id":2739,"date":"2025-01-27T08:43:21","date_gmt":"2025-01-27T07:43:21","guid":{"rendered":"https:\/\/welkerlab.hu\/?page_id=2739"},"modified":"2025-03-07T15:24:14","modified_gmt":"2025-03-07T14:24:14","slug":"bear","status":"publish","type":"page","link":"https:\/\/welkerlab.hu\/en\/bear\/","title":{"rendered":"BEAR"},"content":{"rendered":"
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Base Editor Activity Reporter (BEAR)<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tThe adenine and cytosine base editors (ABE, CBE) have enabled base-pair precision gene editing. BEAR is a plasmid-based reporter system that can be used to test the efficiency of ABE and CBE editors for almost any target sequence. The advantage of the BEAR reporter system is that it can be used to test the editing efficiency of targets independently of the genomic context.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Principle of the base editor activity reporter (BEAR) assay.<\/strong> BEAR consists of a split GFP coding sequence (green) separated by an intron of which splice donor site is altered to \u2018AC\u2019, resulting in a dysfunctional protein (grey). This inactive splice donor site can be rescued either by ABEs reverting the \u2018AC\u2019 splice donor site to \u2018AT\u2019 or by CBEs reverting the \u2018AC\u2019 splice donor site to \u2018GC\u2019, respectively. \u2018AT\u2019 and \u2018GC\u2019 are known to be functional non-canonical splice donor sites in the human genome.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Reference<\/strong>
T\u00e1las, Andr\u00e1s ; Simon, Dorottya Anna ; Kulcs\u00e1r, P\u00e9ter Istv\u00e1n ; Varga, \u00c9va ; Krausz, Sarah Laura ; Welker, Ervin
BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors<\/em>
NATURE COMMUNICATIONS 12 : 1 Paper: 6353 , 14 p. (2021)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tView publication<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Base Editor Activity Reporter (BEAR) Az adenin- \u00e9s citozin- base editorok (ABE, CBE) \u00e1ltal lehet\u0151v\u00e9 v\u00e1lt a b\u00e1zisp\u00e1r pontoss\u00e1g\u00fa g\u00e9nszerkeszt\u00e9s. A BEAR egy plazmid-alap\u00fa riporter rendszer, melynek seg\u00edts\u00e9g\u00e9vel ABE \u00e9s CBE editorok hat\u00e9konys\u00e1ga vizsg\u00e1lhat\u00f3 gyakorlatilag b\u00e1rmilyen target szekvencia eset\u00e9ben. A BEAR riporter rendszer el\u0151nye, hogy a genomi kontextust\u00f3l f\u00fcggetlen\u00fcl vizsg\u00e1lhat\u00f3 benne a targetek edit\u00e1l\u00e1si hat\u00e9konys\u00e1ga. […]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2739","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2739","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/comments?post=2739"}],"version-history":[{"count":42,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2739\/revisions"}],"predecessor-version":[{"id":2903,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/2739\/revisions\/2903"}],"wp:attachment":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/media?parent=2739"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}