{"id":1960,"date":"2024-09-06T16:06:28","date_gmt":"2024-09-06T14:06:28","guid":{"rendered":"https:\/\/welkerlab.hu\/?page_id=1960"},"modified":"2024-10-16T15:07:39","modified_gmt":"2024-10-16T13:07:39","slug":"ligeti-zoltan","status":"publish","type":"page","link":"https:\/\/welkerlab.hu\/en\/ligeti-zoltan\/","title":{"rendered":"Zolt\u00e1n Ligeti"},"content":{"rendered":"
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Zolt\u00e1n Ligeti<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The focus of my current research is the investigation of the target specificity of Cas9 variants in bacterial and mammalian cells. The CRISPR-Cas9 system offers a powerful tool for genetic editing, but different Cas9 variants show different on-target and off-target effects. My goal is to develop a program that allows selection of the optimal Cas9 variant on a given DNA segment, thus minimizing unwanted side effects and maximizing specificity.
With this, I can contribute to increasing the efficiency and safety of precision gene editing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Education<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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2016 - Present<\/em>
Doctoral school
Multidisciplinary Medical Sciences<\/em>
<\/strong>University of Szeged (SZTE)<\/p>

2016<\/em>
Biology MSc
Molecular, Immune and Microbiology specialization<\/em>
<\/strong>E\u00f6tv\u00f6s Lor\u00e1nd University (ELTE)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Research Interest<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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My scientific focus centers on the study of CRISPR-Cas9 variants, diagnostics, and potential therapies for genetic diseases. I have a strong interest in genome editing techniques, particularly those involving the CRISPR-Cas9 system, as well as in the evaluation of next-generation sequencing (NGS) technologies and data, with a focus on genome and transcriptome analysis. Additionally, I am engaged in exploring diagnostics and future therapeutic applications for genetic mutations and rare genetic diseases, including the development and editing of clinical mutation databases. Moreover, I have a keen interest in programming, bioinformatics program development, and the creation of artificial intelligence models. \n
This interdisciplinary approach allows me to bridge genetic research with clinical applications, contributing to advancements in diagnostics and therapeutic interventions.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Publications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Husz\u00e1r, Krisztina\u00a0<\/a>; Welker, Zsombor ;\u00a0Gy\u00f6rgyp\u00e1l, Zolt\u00e1n<\/a>\u00a0;\u00a0T\u00f3th, Eszter<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/strong>\u00a0<\/a>;\u00a0Kulcs\u00e1r, P\u00e9ter Istv\u00e1n\u00a0<\/a>; Dancs\u00f3, J\u00e1nos ;\u00a0T\u00e1las, Andr\u00e1s\u00a0<\/a>;\u00a0Krausz, Sarah Laura\u00a0<\/a>;\u00a0Varga, \u00c9va\u00a0<\/a>;\u00a0Ervin Welker<\/a>
Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs<\/a><\/em><\/span>
NUCLEIC ACIDS RESEARCH 51 : 11 pp. 5847-5863. , 17 p. (2023)
DOI\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed<\/a>
Article (Journal Article)<\/p>

Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a>\u00a0<\/strong>;\u00a0T\u00f3th, Eszter<\/a>\u00a0;\u00a0Rakv\u00e1cs, Zs\u00f3fia<\/a>\u00a0;\u00a0Bartos, Zsuzsanna<\/a>\u00a0;\u00a0Krausz, Sarah Laura<\/a>\u00a0; Welker, Agnes ; V\u00e9gi, Vanessa Laura ;\u00a0Husz\u00e1r, Krisztina<\/a>\u00a0;\u00a0Ervin Welker<\/a>
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets<\/a><\/em><\/span>
NATURE COMMUNICATIONS 14 : 1 Paper: 5746 , 20 p. (2023)
DOI\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed<\/a>
Article (Journal Article)<\/p>

Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a><\/strong>\u00a0;\u00a0Krausz, Sarah Laura<\/a>\u00a0;\u00a0Welker, Ervin\u00a0<\/a>
SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e<\/a><\/em><\/span>
NATURE COMMUNICATIONS 13 : 1 Paper: 6858 , 11 p. (2022)
DOI\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed<\/a>
Article (Journal Article)<\/p>

Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;<\/b>\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0T\u00f3th, Eszter<\/a>\u00a0;\u00a0Nyeste, Antal<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a>\u00a0<\/strong>; Welker, Zsombor ;\u00a0Welker, Ervin<\/a>
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5\u2032G-extended sgRNAs<\/a><\/em><\/span>
NATURE COMMUNICATIONS 11 : 1 Paper: 1223 , 14 p. (2020)
DOI\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed<\/a>
Article (Journal Article)<\/p>

T\u00f3th, Eszter<\/a>\u00a0;\u00a0Varga, \u00c9va<\/a>\u00a0;\u00a0Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0; Kocsis-Jutka, Vir\u00e1g ;\u00a0Krausz, Sarah Laura<\/a>\u00a0;\u00a0Nyeste, Antal<\/a>\u00a0; Welker, Zsombor ;\u00a0Husz\u00e1r, Krisztina<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a><\/strong>\u00a0;\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Welker, Ervin<\/a>
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases<\/a><\/em><\/span>
NUCLEIC ACIDS RESEARCH 48 : 7 pp. 3722-3733. , 12 p. (2020)
DOI\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed\u00a0 \u00a0<\/a>Other URLs<\/a>
Article (Journal Article)<\/p>

T\u00f3th, Eszter<\/a>\u00a0; Czene, Bernadette C ;\u00a0Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;\u00a0Krausz, Sarah Laura<\/a>\u00a0;\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Nyeste, Antal<\/a>\u00a0;\u00a0Varga, \u00c9va<\/a>\u00a0;\u00a0Husz\u00e1r, Krisztina<\/a>\u00a0;\u00a0Weinhardt, N\u00f3ra<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a>\u00a0<\/strong>;\u00a0Borsy, Adrienn \u00c9va<\/a>\u00a0;\u00a0Fodor, Elfrieda<\/a>\u00a0;\u00a0Welker, Ervin<\/a>
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants<\/a><\/em><\/span>
NUCLEIC ACIDS RESEARCH 46 : 19 pp. 10272-10285. , 14 p. (2018)
DOI\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed<\/a>
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Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;\u00a0T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Husz\u00e1r, Krisztina<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a><\/strong>\u00a0;\u00a0Toth, Eszter<\/a>\u00a0;\u00a0Weinhardt, N\u00f3ra<\/a>\u00a0;\u00a0Fodor, Elfrieda<\/a>\u00a0;\u00a0Welker, Ervin<\/a>
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage<\/a><\/em><\/span>
GENOME BIOLOGY 18 Paper: 190 , 17 p. (2017)
DOI\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed\u00a0 \u00a0<\/a>\u00a0SZTE Publicatio<\/a>
Article (Journal Article)<\/p>

T\u00e1las, Andr\u00e1s<\/a>\u00a0;\u00a0Kulcs\u00e1r, P\u00e9ter Istv\u00e1n<\/a>\u00a0;\u00a0Weinhardt, N\u00f3ra<\/a>\u00a0;\u00a0Borsy, Adrienn<\/a>\u00a0;\u00a0T\u00f3th, Eszter<\/a>\u00a0;\u00a0Szeb\u00e9nyi, Korn\u00e9lia<\/a>\u00a0;\u00a0Krausz, Sarah Laura<\/a>\u00a0;\u00a0Husz\u00e1r, Krisztina<\/a>\u00a0;\u00a0Vida, Istv\u00e1n<\/a>\u00a0;\u00a0Sturm, \u00c1d\u00e1m<\/a>\u00a0; Gordos, Bianka ;\u00a0Hoffmann, Orsolya Ivett\u00a0<\/a>;\u00a0Bencsura, Petra<\/a>\u00a0;\u00a0Nyeste, Antal<\/a>\u00a0;\u00a0Ligeti, Zolt\u00e1n<\/a><\/strong>\u00a0;\u00a0Fodor, Elfrieda<\/a>\u00a0;\u00a0Welker, Ervin<\/a>
A convenient method to pre-screen candidate guide RNAs for CRISPR\/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid<\/a><\/em><\/span>
DNA RESEARCH 24 : 6 pp. 609-621. , 13 p. (2017)
DOI\u00a0 \u00a0<\/a>\u00a0REAL\u00a0 \u00a0<\/a>WoS\u00a0 \u00a0<\/a>\u00a0EDIT\u00a0 \u00a0<\/a>Scopus\u00a0 \u00a0<\/a>PubMed\u00a0 \u00a0<\/a>\u00a0SZTE Publicatio<\/a>
Article (Journal Article)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Ligeti Zolt\u00e1n Predoc Jelenlegi kutat\u00e1som k\u00f6z\u00e9ppontj\u00e1ban a Cas9 vari\u00e1nsok targetspecifit\u00e1s\u00e1nak vizsg\u00e1lata \u00e1ll bakteri\u00e1lis \u00e9s eml\u0151s sejtekben. A CRISPR-Cas9 rendszer hat\u00e9kony eszk\u00f6zt k\u00edn\u00e1l a genetikai szerkeszt\u00e9sekhez, de a k\u00fcl\u00f6nb\u00f6z\u0151 Cas9 vari\u00e1nsok elt\u00e9r\u0151 on-target \u00e9s off-target hat\u00e1sokat mutatnak. C\u00e9lom egy olyan program kifejleszt\u00e9se, amely lehet\u0151v\u00e9 teszi az optim\u00e1lis Cas9 vari\u00e1ns kiv\u00e1laszt\u00e1s\u00e1t egy adott DNS r\u00e9szen, minimaliz\u00e1lva ezzel […]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-1960","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/1960","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/comments?post=1960"}],"version-history":[{"count":70,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/1960\/revisions"}],"predecessor-version":[{"id":2707,"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/pages\/1960\/revisions\/2707"}],"wp:attachment":[{"href":"https:\/\/welkerlab.hu\/en\/wp-json\/wp\/v2\/media?parent=1960"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}